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mosquito® LCP

Automated membrane protein screening, optimization and scale-up

mosquito LCP overcomes the common problems encountered with accurately dispensing the highly viscous LCP mixture used in membrane protein crystallization. It allows you to fully automate LCP set-ups accurately and repeatedly.

Incredible power at the heart of your liquid handling processes

reduced set-up time and training requirements

walk-up and use technology which even novice users can master quickly

robust day-to-day performance even in heavy use

proven reliability with no blocking or clogging

protein samples go further and screen consumption is vastly reduced

access volumes as low as 10 nL on multi-aspirate

faster screening of crystallization space for less sample

rapid and accurate drop set-up across a wide viscosity range

flexibility to easily switch methods to suit individual projects

easily set all crystallization methods with no configuration changes

accuracy and precision, irrespective of liquid class

true positive displacement pipetting

Automated lipidic cubic phase screening

mosquito LCP allows you to dispense lipidic cubic phase (LCP) volumes as low as 25 nL, while automated calibration of syringe and pipette positioning ensures precise drop-on-drop placement for easy automated imaging. In addition, mosquito LCP includes all the functionality of mosquito crystal for your automated vapour diffusion set-ups. This allows you to set up both LCP and traditional protein crystallization experiments in any commercially available plate, using just one instrument with no configuration changes, additional hardware or software.

Perfect drops for protein crystallization

mosquito’s unique nanolitre liquid handling technology can perform multiple aspirations before a single dispense, which is essential for automating seeding and direct addition of additive screens to the drops. You can even dispense a combination of solutions simultaneously, with additional mixing if required, ensuring perfect drop formation for optimal protein crystallization.

Innovation

Humidity control at your fingertips with up to a 90% reduction in drop evaporation

Sample evaporation can be a problem when dispensing very low volumes or volatile samples, causing inconsistent drop volumes, especially when environmental conditions and local humidity vary. mosquito’s active humidity chamber reduces experimental inconsistencies by allowing users to control the relative humidity (RH) of each experiment.
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We're revolutionizing liquid handling

Our liquid handling portfolio provides you with accurate and repeatable nanolitre to microlitre pipetting, every time, irrespective of liquid viscosity or environmental conditions.

Built for performance

Our liquid handling instruments use innovative technologies within products to provide real world benefits to researchers.

We produce robust, reliable and easy to use products that overcome difficult technical challenges so that you can focus on solving the scientific ones.

Pipetting technology

Each of our disposable micropipettes has its own individual piston, not an air gap or system liquid, offering true positive displacement pipetting with no risk of cross-contamination.

Humidity control at your fingertips with up to a 90% reduction in drop evaporation

Sample evaporation can be a problem when dispensing very low volumes or volatile samples, causing inconsistent drop volumes, especially when environmental conditions and local humidity vary. mosquito’s active humidity chamber reduces experimental inconsistencies by allowing users to control the relative humidity (RH) of each experiment.

jpg of humidity chamber

Intuitive by design

Our software is as focused on usability as it is on functionality. We strive for an intuitive user experience that matches the requirements of each scientific application. Through collaboration with scientists, our software team develop and enhance the instrument UI to provide the optimal user experience and facilitate adoption into novel application areas.

mosquito software

Enhance your protein crystallization research

We also offer additional components to enhance your membrane protein crystallization screening, such as a humidity chamber and an automated mixer for preparing LCP.

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jpg of the spool cover

Featured applications

mosquito liquid handlers have been integrated with a wide variety of other laboratory instrumentation, including robotic plate handlers, stackers and bulk liquid handlers, to achieve walk-away operation in many application areas.

In-meso (LCP) screen set-up

The in-meso (Lipidic Cubic Phase or LCP) crystallization technique, employs highly viscous lipid mesophases to contain the membrane proteins for crystallization and has revolutionized membrane protein crystallography.

Bicelle setup

mosquito can automate the bicelle method of membrane protein crystallization. By maintaining a cold environment and pre-chilling the sample holder block it is possible to use mosquito crystal to accurately and easily pipette the bicelle solution, thereby facilitating the automation of screening for this lipidic based technique of membrane protein crystallization.

Sitting drop

For sitting drop, mosquito LCP’s precise pipetting ensures accurate drop-on-drop placement in the centre of sub-wells for perfect automated imaging, whilst avoiding drops wicking up the well walls.

Hanging drop

mosquito LCP makes automating hanging set-ups easy, as it can mirror the screen drops onto the plate seal which is then inverted and sealed over the wells.

Microseeding

Seeding or micro matrix seeding (MMS) is a well-established method used to improve the quality and reproducibility of protein crystals. mosquito's plate set-up time is less than 3 minutes to create multiple aspirations before pipetting to ensure that no protein or seed stock is wasted.

Microbatch screen set-up

mosquito LCP ensures that screen and protein drops always combine because both protein and screen solutions can be aspirated consecutively within the same tip and then to be pipetted directly through oil.

Additive screening

mosquito's unique true positive displacement pipettes combined with its multi-aspirate function allows users to access volumes down to 10 nL. Not only does this save on precious ligands/compounds, the contact dispensing ensures precise placement within the crystallization drops.

Crystal soaking

Soaking protein crystals with ligands is the fastest route to produce high-throughput structures for fragment based drug discovery. mosquito can be used to prepare small soaking drops of DMSO based fragment libraries directly from stock plates.

Technical specifications

Pipetting range
25 nL - 1.2 μL
Throughput
2 minutes/ standard 96-well plate, 4 minutes/ 96-well
LCP plate
Primary SBS plate format
48, 96 and 384
Accessories
Active humidity chamber, spool cover and LCP mixer
Weight
34 kg (75 lbs)
Dimensions (W x D x H)
440 mm (790 mm 4 plate deck) x 470 mm x 690 mm
17.3 in (31 in) x 18.5 in x 27 in
Technical variants
2 or 4 plate position deck
What our customers say

What our customers say

The mosquito LCP is our workhorse instrument for the accurate and reliable dispensing of sub-microliter droplet from aqueous and LCP solutions. Reproducibility is key to what we do and the mosquito certainly delivers with excellent speed. Service support is critical to ensuring maximum use of the instrument and the team at TTP Labtech is extremely flexible and deliver to our high expectations.

Paul McEwan

Evotec (UK) Ltd.

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