Automated membrane protein screening, optimisation and scale-up
reduced set-up time and training requirements
walk-up and use technology which even novice users can master quickly
robust day-to-day performance even in heavy use
proven reliability with no blocking or clogging
protein samples go further and screen consumption is vastly reduced
access volumes as low as 10 nL on multi-aspirate
faster screening of crystallisation space for less sample
rapid and accurate drop set-up across a wide viscosity range
flexibility to easily switch methods to suit individual projects
easily set all crystallisation methods with no configuration changes
accuracy and precision, irrespective of liquid class
true positive-displacement pipetting
Automated lipidic cubic phase screening
mosquito LCP allows you to dispense lipidic cubic phase (LCP) volumes as low as 25 nL, while automated calibration of syringe and pipette positioning ensures precise drop-on-drop placement for easy automated imaging. In addition, mosquito LCP includes all the functionality of mosquito crystal for your automated vapour diffusion set-ups. This allows you to set up both LCP and traditional protein crystallisation experiments in any commercially available plate, using just one instrument with no configuration changes, additional hardware or software.
Perfect drops for protein crystallisation
mosquito’s unique nanolitre liquid handling technology can perform multiple aspirations before a single dispense, which is essential for automating seeding and direct addition of additive screens to the drops. You can even dispense a combination of solutions simultaneously, with additional mixing if required, ensuring perfect drop formation for optimal protein crystallisation.
Humidity control at your fingertips with up to a 90% reduction in drop evaporation
Built for performance
Our liquid handling instruments use innovative technologies within products to provide real world benefits to researchers.
We produce robust, reliable and easy to use products that overcome difficult technical challenges so that you can focus on solving the scientific ones.
Each of our disposable micropipettes has its own individual piston, not an air gap or system liquid, offering true positive-displacement pipetting with no risk of cross-contamination.
Intuitive by design
Our software is as focused on usability as it is on functionality. We strive for an intuitive user experience that matches the requirements of each scientific application. Through collaboration with scientists, our software team develop and enhance the instrument UI to provide the optimal user experience and facilitate adoption into novel application areas.
Enhance your protein crystallisation research
We also offer additional components to enhance your membrane protein crystallisation screening, such as a humidity chamber and an automated mixer for
In-meso (LCP) screen set-up
The in-meso (Lipidic Cubic Phase or LCP) crystallisation technique, employs highly viscous lipid mesophases to contain the membrane proteins for crystallisation and has revolutionised membrane protein crystallography.
mosquito can automate the bicelle method of membrane protein crystallisation. By maintaining a cold environment and pre-chilling the sample holder block it is possible to use mosquito crystal to accurately and easily pipette the bicelle solution, thereby facilitating the automation of screening for this lipidic based technique of membrane protein crystallisation.
For sitting drop, mosquito LCP’s precise pipetting ensures accurate drop-on-drop placement in the centre of sub-wells for perfect automated imaging, whilst avoiding drops wicking up the well walls.
mosquito LCP makes automating hanging set-ups easy, as it can mirror the screen drops onto the plate seal which is then inverted and sealed over the wells.
Seeding or micro matrix seeding (MMS) is a well-established method used to improve the quality and reproducibility of protein crystals. mosquito's plate set-up time is less than 3 minutes to create multiple aspirations before pipetting to ensure that no protein or seed stock is wasted.
Microbatch screen set-up
mosquito LCP ensures that screen and protein drops always combine because both protein and screen solutions can be aspirated consecutively within the same tip and then to be pipetted directly through oil.
mosquito's unique true positive displacement pipettes combined with its multi-aspirate function allows users to access volumes down to 10 nL. Not only does this save on precious ligands/compounds, the contact dispensing ensures precise placement within the crystallisation drops.
17.3 in (31 in) x 18.5 in x 27 in
What our customers say
The mosquito LCP is our workhorse instrument for the accurate and reliable dispensing of sub-microliter droplet from aqueous and LCP solutions. Reproducibility is key to what we do and the mosquito certainly delivers with excellent speed. Service support is critical to ensuring maximum use of the instrument and the team at TTP Labtech is extremely flexible and deliver to our high expectations.
Related products, accessories and consumables
The very best technical assistance for mosquito LCP from our dedicated service and support team.
Backed by our highly committed service team, our mosquito LCP service contracts ensure peak performance for complete peace of mind.
Dedicated in-house scientists supporting mosquito LCP application and assay enquiries.
Expert training gets you up and running in no time and ensures best practise, ensuring peak performance from your mosquito LCP.
Interested? Get in touch to start the journeyAsk an expert