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Saving time and money – high content imaging for pre-clinical cell health screening

Our blog

01 October, 2013

Our latest webinar explores the best ways to effectively leverage high content imaging (HCI) drug screening for avoiding costly drug failures during clinical trials – now available to watch ‘on demand’ on our website.

30% of the drug failures observed during clinical testing are due to unexpected toxicity. Fortunately, high throughput, HCI at the early drug screening stage can be used to identify these compounds. This enables them to ‘fail early,’ saving time and money.

Our recent webinar illustrated how to multiplex a number of cell health assays to take full advantage of the benefits offered by HCI cell health screening.

‘Multiplexing quickly’

Even though drug screening relies upon rapid and cost-effective assays, achieving high throughput has traditionally been challenging for those working with HCI. There are many possible reasons for this, but the majority revolve around bottlenecks in image analysis.

For example, many HCI systems work by capturing a series of distinct images of a well, which are then stitched together to form a complete picture. Unfortunately, this takes time and computing power and can lead to unwanted image artefacts. This is bad news for both throughput and accuracy. Multiplexing just multiplies the problem – the more markers used, the more images required, the more complex the analysis process!

To sidestep these issues, engineering innovations are improving the process by enabling fast whole well imaging without the need to capture and stitch together separate images.

By watching our latest webinar, you’ll learn about specific cell health assays including several ways to detect apoptosis and how these can be multiplexed to optimise your cell health screening studies.

Get all your questions answered…

If you want to know how to effectively use HCI in your toxicity screening workflow, while avoiding many of the common pitfalls of the technique our webinar addresses these issues along with many of the questions that you may have:

  • How fast can I screen a plate of samples?
  • Can I boost the efficiency of my work by minimising sample preparation steps?
  • Are there ways to lower the cost of my assays?
  • What should I be thinking about when creating a multiplex assay?
  • I need assay flexibility – is it possible to carry out live/dead assays and detect necrosis vs apoptosis using a single system?
  • How can I generate open source TIFF files for further analysis using my favourite software?