Webinar: a crystallization strategy focused on a high throughput seeding technique
In this webinar recording, Dr Alexey Rak, Sanofi R&D, gives:
- an introduction to MMS technology and the principles of microseeding
- statistical examples of the crystallization of small molecules-protein complexes and antibody-antigen complexes
- conclusions about the time and cost-effectiveness of using microseeding in ambitious structural biology labs.
Modern drug discovery processes require a cost and time-effective operation from structural biology teams to provide non-ambiguous solutions and support for structure-based rational drug design projects. Protein crystallization success rates and improvements in crystallization optimization time can positively influence drug discovery.
In Sanofi’s R&D labs, Dr Rak achieves this by applying microseeding crystallization techniques assisted by the mosquito robot (TTP Labtech). Together, these have been demonstrated to improve the quality of protein crystals and are used instead of, or in concert with, routine crystallization optimization. Microseeding is applied to the generation of new ligand bound crystal forms as well as to crystallizing proteins and protein complexes which were not otherwise possible to crystallize.