Tutorial: miniaturization and automation of single-cell RNA-seq library preparation at UCSD
mosquito HV genomics,
mosquito LV genomics
In this tutorial, Dr Louise Laurent (UC San Diego) demonstrates how Nextera XT library preparation can be scaled down to as little as 2 µL total volume, using the accurate positive displacement pipetting technology of a mosquito liquid handler in an automated high-throughput environment. The workflow, has been published in the Journal of Laboratory Automation.
This tutorial covers:
- high-throughput, low-cost preparation of single cell RNA-seq libraries with up to 90% cost saving
- validation of low-volume libraries against kit manufacturers’ recommended volumes
- low-volume and low-cost magnetic bead clean up
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