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simultaneous determination of antibody binding, specificity and titre on the mirrorball fluorescence cytometer.

mirrorball, 

sol-R reagents

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The antibody discovery process relies on consecutive screens to test the same antibody sample for binding affinity, target specificity and antibody titre. Conventionally such screens are done by ELISA or flow cytometry, but both techniques have technical limitations that restrict their utility in high-throughput screening environments:

  • multiple wash steps are required, with significant hands-on time from the operator restricting throughput
  • ELISA assays generate only singleplex readouts
  • sample throughput rate and plate capacity of flow cytometers is incompatible with screening environments

Here we present a simple no-wash assay to screen antibody samples for binding to a target cell line, non-specific binding to a control cell line and antibody titre by binding to a bead.