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High-throughput, low-volume soaking of protein crystals in the rapid screening of fragment libraries

mosquito Xtal3, 

mosquito crystal, 

mosquito LCP

Posters

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Growing protein-ligand complex crystals can be challenging, especially in cases where the affinity is poor and the solubility of the ligand in the crystallization condition is low. Various methodologies are often trialled before obtaining a diffraction-quality protein-ligand crystal.

Co-crystallization is a common method for producing protein-ligand complex structures. It is especially useful when drug-like compounds trigger conformational changes in proteins. This can result in variations in the growing conditions or crystal forms, and may necessitate wider screening strategies for co-crystallization in general.

Alternatively, soaking protein crystals with ligands is the fastest route to produce highthroughput structures, as long as the starting crystal form is easy to grow reproducibly, able to accommodate the desired ligand and, is robust to physical and chemical changes.

This poster will describe a low-volume, highthroughput soaking method developed by Dr. David Hargreaves (AstraZeneca, UK) for screening a fragment-based lead generation (FBLG) library. TTP Labtech’s mosquito® crystal enabled fast and accurate miniaturization of the crystallization set-up.