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Miniaturization of Illumina Library Preparation in a Shared Facility Setting

mosquito HV genomics, 

mosquito LV genomics

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The high cost of Illumina library production is a critical factor in the utilisation of underpowered experiments. Several studies have demonstrated that increasing biological replicates, even without increasing the total amount of sequencing, significantly improves experimental power. Automated liquid handlers can address this issue thought increased throughput and miniaturisation of reaction volumes. However, integrating these robots in core facilities can be challenging due to the diverse projects, sample types, and sample qualities found in shared facilities. Here we demonstrate the miniaturisation of a diverse set of Illumina library protocols on the TTP Labtech mosquito HV and their application to a broad variety of biological sample types. Methods for both RNA and DNA library production and from intact and fragmented samples have been successfully implemented and are widely used. The miniaturisation of these protocols has resulted in both significant savings in library production cost, and in a notable increase in average number of replicates performed by the scientists.