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A practical solution for phenotypic screening of a full compound library

acumen Cellista

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Phenotypic screening has become ever more prevalent over the last decade, but their use with primary screening
campaigns is not a trivial exercise. Key factors include the culture of large numbers of cells, their plating into microplates, and the complexity of the assay protocol being applied. Most high content imaging systems also offer limited capability for primary screening of full compound libraries due to their low throughput with high-density microplates, large data files and long image analysis times, making them impractical to deploy such systems for full deck library high-throughput screening. As a result when using this approach, a compromise is taken to screen only much smaller library subsets. Such a targeted approach reduces the power of using a phenotypic screen by limiting the chemical diversity being investigated.

Laser-scanning imagers, such as the acumen, combine the object-recognition capabilities of microscope based systems with the fast read speeds of bulk fluorescence readers (typically under 5 minutes per plate) which are compatible with
primary full deck compound library screening campaigns. 96-, 384- and 1,536- well microplates can all be analysed at
equivalent plate read times since imaging is performed on an area and not well basis. Here we discuss the requirements for a full deck phenotypic screen and describe how the acumen could be employed in a high-throughput, full deck phenotypic screen, with capacities up to over 400,000 wells/screening day.