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Rapid analysis of 3D cultures in soft agar and on ultra-low attachment plates using a laser scanning imaging system

acumen Cellista

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Here we describe the formation of such spheroids on ultra-low attachment plates and colonies in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats. We then used the acumen to rapidly image entire microplates (5 minutes/plate), reporting a range of parameters such as spheroid number, area and volume. The acumen is ideally suited to the high content analysis of spheroids, as the whole-well scanning capability of the instrument will include data from all the spheroids in a well, while the large depth of field of the scan lens allows the determination of individual spheroid volume without the need to acquire a Z-stack of images.