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Multiplexed fluorescence ELISA assays on mirrorball

mirrorball, 

sol-R reagents

Application notes

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Although ELISAs are very sensitive, they are time-consuming, require multiple separate wash steps and incubation steps and are not amenable to multiplexing. In this application note, we demonstrate the transfer of standard commercially available colorimetric IL-8 and IL-6 ELISA kits onto a no-wash fluorescence based ELISA on the mirrorball. The resulting ELISA format offers significant process efficiency improvements through use of a single incubation step, without the requirement for wash steps, and is amenable to multiplexing.