Laser scanning imaging cytometry: redesigning cytometry into a format fit for screening
Addressing market needs through innovative product design
TTP Labtech has collaborated with cell biologists since 1997. This enabled us to spot a market opportunity early; a need for a screening system that was as fast as a fluorescence plate reader but delivered higher content cell-based data. In 2000 the first generation acumen® (now in its 4th generation) was developed with laser scanning imaging cytometry (LSIC) at the core of the product design, followed by mirrorball® in 2010 to address unmet needs within immunoassay screening.
Designed for discovery: how our LSIC approach meets the needs of screening
Our LSIC systems bring the benefits of cytometry to the screening environment. Through unique optical design, plus an intelligent approach to data acquisition and analysis, our systems deliver:
- walk-up and use simplicity (no routine maintenance, or cleaning)
- system and data reliability
- individual object recognition
- fast data acquisition
- multichannel detection
- easy to learn software
- small data file generation through smart thresholding
- simple integration with automation
Removing limitations through purpose-built design
acumen Cellista: single point laser scanning through a specialised high depth-of-collection f-theta lens and photomultiplier tube (PMT) signal detection. Enables rapid whole-well imaging of cells and large 3D objects labelled with up to 4 multiplexed fluorophores over an area of 18 x 18 mm.
mirrorball: single point laser scanning system with an aperture positioned close to a confocal image plane to reject background fluorescence, enabling homogenous assays. The optical path is optimised to reduce light loss for sensitivity and designed to allow the simultaneous capture of several emissions from fluorophores using PMT detection.
acumen Cellista; bringing physiological relevance to screening without bottlenecks
acumen Cellista is designed to deliver greater content than traditional plate readers, but at similar throughputs. It brings improved physiological relevance to high throughput screens through the use of multiplexed cell-based assays from which decision making data can be gained in as little as 2.5 minutes per microplate. This is achieved through an image-based approach that is uniquely approachable for HTS departments, as there’s no requirement for specialist operators nor mountains of data to manage.
mirrorball: removing limitations to expand the possibilities
mirrorball is designed to address the process inefficiencies of ELISA protocols and the throughput challenges of flow cytometry. It’s narrow depth of field imaging enables laborious singleplex ELISA protocols to be streamlined into no-wash, multiplex formats that significantly improve productivity. mirrorball’s no fluidic design permits reliable walk-away automation for
walk-up and use HTS friendly technology
simple to operate and simple to integrate
enable immediate data driven decisions
fast read and analysis times
meet project deadlines
technology and data you can depend upon
multiplex and multi-application breadth
work the way you want to
designed to save
do more with less reagent, time and budget
effortless throughput for cytometry applications
Workflow efficiency for sandwich ELISA
A simple two-step reagent addition protocol is all that is required from a mirrorball no-wash, multiplexed sandwich immunoassay that won’t bust your budget.
Accelerating the pace and reliability of antibody discovery
Escape the constraints of traditional ELISA and flow cytometry assays. Choose the most relevant model (suspension, adherent cells or beads) for your target, then multiplex hit identification and counter-screens into one productive no-wash immunoassay.
Cell-based HTS without compromise
Combine biologically relevant cell-based assays and content rich multiplexing at HTS speeds to ensure you never miss that hit. Featured applications include: cell viability, 3D spheroid imaging, genome-wide phenotypic screening and viral infectivity.
Multiplexed cell-based assays for hit confirmation
Relieve the pressure from microscope-based technologies. Transfer routine hit confirmation screens to a complementary solution and instantly eliminate resource and throughput bottlenecks. Featured applications include: cell health profiling, kinase activation, angiogenesis and cell cycle.