Efficient high-throughput NGS sample preparation
reproducible results from miniaturised reactions and assays
pinpoint accuracy at low volumes
increase productivity and throughput
rapid programming and high speed plate setup
generate robust, reliable data
disposable, sterilised tips ensure zero cross-contamination
develop your own protocols with ease
open system that aspirates, dispenses and mixes
reduce reagent consumption and generate more results from your budget
miniaturise costly processes and reduce dead volumes
accurate even with highly viscous liquids
unique positive displacement pipetting technology
NGS library preparation
Reducing the volume of NGS library preparation by 5-fold to 25-fold with mosquito leads to dramatic cost savings. A wide range of kits can be miniaturised, for example robust Nextera XT library preparation in only a few microlitres total volume has been demonstrated.
Magnetic bead clean-up
With mosquito® HV genomics, nucleic acids can be cleaned up and size-selected with magnetic beads at high throughput, which complements miniaturised library preparation processes. A 384-well magnetic block has been specifically designed for this purpose.
DNA normalisation and pooling
NGS protocols often require the concentration of input DNA or the sequencing libraries to be normalised. Single-channel mosquito® X1 delivers highly accurate normalisation and pooling in the nanolitre to low microlitre volume range.
High-throughput DNA quantification
mosquito® LV genomics is uniquely suited to the setup of DNA quantification assays in a high-throughput production genomics environment. Accurate and reliable nanolitre handling even of viscous genomic DNA leads to highly reproducible results from only a few hundred nanolitres of DNA sample.
A versatile open platform for miniaturised NGS library preparation
Sequencing technology evolves rapidly. As new improved protocols and kits emerge, it is crucial to keep lab processes up to date. mosquito® is a liquid handler compatible with standard SBS plates, using an open software format that makes it exceptionally easy to program your own protocols. mosquito provides any genomics lab or core facility with the power and flexibility to stay cutting-edge for many years to come.
The technology: positive displacement pipetting
Unlike traditional liquid handlers, mosquito liquid handling robots use positive displacement pipetting technology. This superior pipetting mechanism allows transfers in the 25 nL – 5 µL range with high precision and accuracy. Importantly, it offers the ability to pipette even liquids with high viscosities, such as enzymes in 50% glycerol or genomic DNA, accurately and precisely. Furthermore, the use of disposable mosquito tips ensures gentle sample handling and zero cross-contamination.
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