Protein Crystallization using mosquito
TTP LabTech’s mosquito® Crystal and LCP are the protein crystallographer’s favourite liquid handlers. They make protein crystallization screening faster, more cost-effective and quite simply easier than ever before.
Protein crystallization
With mosquito® Crystal you can use smaller volumes of precious protein sample with no risk of cross contamination, resulting in cost savings and allowing more extensive screening. You can automate all the popular protein crystallization techniques; hanging drop, sitting drop, microbatch as well as seeding or additive screening plate preparation, without the need to make setup changes to the instrument.
mosquito’s accuracy and repeatability means you can place drops centrally in the sub-wells of sitting-drop plates every time. Precise drop placement facilitates automated analysis, thus making your protein crystals easier to identify.
TTP LabTech’s mosquito Crystal also allows you to create several multi-component drops per well, even in 96-well hanging drop set-ups so you can assess different protein concentrations at the same time. mosquito's unique nanolitre liquid handling technology can perform multiple aspirations before a single dispense, which is essential for automating additive screening. You can even dispense a combination of solutions simultaneously – with additional mixing if required – ensuring perfect drop formation for optimal protein crystallization.
Lipid Cubic Phase (LCP) Screening
Membrane proteins are difficult to purify and crystallize as they require highly viscous lipids or detergents in order for them to retain their true structure. The use of TTP LabTech's mosquito® LCP for membrane protein crystallization screening overcomes the common problems encountered with accurately dispensing nanolitre quantities of these viscous lipids. mosquito LCP allows you to dispense LCP volumes as low as 25nL, while automated calibration of syringe and pipette positioning ensures precise drop-on-drop placement for easy automated imaging.
In addition, mosquito LCP includes all the functionality of mosquito Crystal for your automated vapour diffusion set-ups, allowing you to set up both LCP and traditional protein crystallization experiments in any commercially available plate.
mosquito LCP employs our proven positive displacement mosquito tips for screen additions offering guarantee of zero cross-contamination.
TTP LabTech also offer additional components to enhance your membrane protein crystallization screening, such as an optional humidity cover and an automated mixer for preparing LCP.
For further information or to request a demonstration in your lab please contact us or fill in the form.