Bibliography
A selection of published posters and articles using TTP LabTech’s range of Acumen laser scanning microplate cytometers are outlined below. The PubMed links are given, where appropriate.
POSTERS
2009
A high throughput screening homogenous antibody binding assay using the Acumen eX3
High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry
A novel multiplexed-compatible high throughput cell migration screening assay using an Acumen eX3
2008
HIgh content image analysis using a laser scanning microplate cytometer
Use of far red emitting DNA dye DRAQ5 for cell cycle analysis with microplate cytometry
Label-free analysis of tissue sections and cells using laser scanning reflectance imaging
A high content homogneous assay for P-glycoprotein inhibition using microplate cytometry
A heterogenous angiogenesis assay using primary cell co-culture in microchannels
Analysis of kinase inhibitors using a large array of signaling antibodies and high content screening
2007
Developing cell-based fluorescent assays for laser scanning microplate cytometry
Cell proliferation and cell cycole analysis using a multiple laser microplate cytometer
High content screening in oncology research using an Acumen eX3
HIgh throughput screening using a multiple laser Acumen eX3 microplate cytometer
Cell proliferation and cell cycle analysis using a multiple laser microplate cytometer
Cell cycle analysis using an Acumen eX3 microplate cytometer
2006
An ultra-high throughput approach to high content screening in 1536-well format
2005
High throughput profiling of siRNA and miRNA libraries using microplate cytometry
HIgh throughout automated high content screening using a BioCube system and microplate cytometry
High content screening of red blood cell differentiation and cytotoxicity using an Acumen Explorer
2004
Determination of protein translocation using a novel variation on the watershed algorithm
Multicolour detection of CD45 on human monocytes iwth quantum dots in a high-throughout format
A high content primary screening assay determining ERK adn JNK activation
ARTICLES
2010
Chresta et al. AZD8055 is a potent, selective, and orally bioavailable ATP-competitive mammalian target of rapamycin kinase inhibitor with in vitro and in vivo antitumor activity. Cancer Res. 2010;70(1):288-98
Hickinson et al. AZD8931, an Equipotent, Reversible Inhibitor of Signaling by Epidermal Growth Factor Receptor, ERBB2 (HER2), and ERBB3: A Unique Agent for Simultaneous ERBB Receptor Blockade in Cancer. Clin Cancer Res. 2010;16:1159-69.
Fuchs et al. Clustering phenotype populations by genome-wide RNAi and multiparametric imaging. Molecular Systems Biology . 2010; 6: Article number 370
Karlas et al. Genome-wide RNAi screen identifies human host factors crucial for influenza virus replcation. Nature 2010;463:818-822
Lees et al. P2X7 receptor-mediated killing of an intracellular parasite, toxoplasma gondii by human and murine macrophages. J Immunol 2010; 184:7040-6
Phong et al. The p38 MAPK promotes cell survival in response to DNA damage but is not required for G2 DNA damage checkpoint in human cancer cells. Mol Cell Biol. Jun 1 in press
2009
Brimacombe et al.A dual-fluorescence high-throughput cell line system for probing multidrug resistance. Assay Drug Dev Technol. 2009;7:233-49.
Dessole et al. Discovery of N-[(1-aryl-1H-indazol-5-yl)methyl]amides derivatives as smoothened antagonists for inhibition of the hedgehog pathway. Bioorg Med Chem Lett. 2009; 19:4191-5
Forte & Luftig MDM2-Dependent Inhibition of p53 Is Required for Epstein-Barr Virus B-Cell Growth Transformation and Infected-Cell Survival. Journal of Virology 2009 83(6):2491-2499
Kajiyama et al. Tetracycline-inducible gene expression in conditionally immortalized mouse podocytes. Am J Nephrol 2009;29:153-163
Klinghoffer et al. A Lentivirus-Mediated Genetic Screen Identifies Dihydrofolate Reductase (DHFR) as a Modulator of b-Catenin/GSK3 Signaling. PLoS One. 2009;4(9). E6892
Malony et al. Induction of thrombospondin-1 partially mediates the anti-angiogenic activity of dexrazoxane. British Journal of Cancer 2009;101:957–966
Nevo et al. Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance. Clin Cancer Res. 2009;15:6570-81
Salmela et al. Dietary flavonoid fisetin induces a forced exit from mitosis by targeting the mitotic spindle checkpoint. Carcinogenesis 2009;30:1032–1040
Swanton et al. Chromosomal instability determines taxane response. Proc Natl Acad Sci U S A. 2009;106:8671-6.
2008
Baus et al. Identification of a novel AS160 splice variant that regulates GLUT4 translocation and glucose-uptake in rat muscle cells. Cell Signal 2008;20:2237-46
Beller et al. COPI complex is a regulator of lipid homeostasis. PloS Biology 2008;6:e292
Lehto et al. The R-Ras interaction partner ORP3 regulates cell adhesion. Journal of Cell Science 2008;121:695-705
Michael et al. A Robotic Platform for Quantitative High Throughput Screening. Assay Drug Dev Technol. 2008;6:637–657
Schmidt et al. A Novel Splicing Variant of Proprotein Convertase Subtilisin/Kexin Type 9. DNA and Cell Biology. 2008;27:183–189
Vuoriluoto et al. Syndecan-1 supports integrin α2β1-mediated adhesion to collagen. Exp Cell Res. 2008;314:3369-81
2007
Kittler et al. Genome-scale RNAi profiling of cell division in human tissue culture cells. Nature Cell Biology 2007;9:1401-12
Qian et al. Secreted PCSK9 downregulates low density lipoprotein receptor through receptor-mediated endocytosis. J Lipid Res 2007;48:1488-98
Wylie & Bowen. Determination of cell colony formation in a high-content screening assay. Clin Lab Med 2007;27:193-9
2006
Auld et al. Fluorescent Protein-Based Cellular Assays Analysed by Laser Scanning Cytometry in 1536-Well Format. Methods in Enzymology 2006;414:566-589.
Bowen & Wylie. Application of laser-scanning fluorescence microplate cytometry in high content screening. Assay and Drug Development Technologies. 2006;4: 209-221.
Kriauciunas & Roell. A Perspective from Eli Lilly and Co, European Pharmaceutical Review, 2006;11:42-51.
Pellinen et al. Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins. J Cell Biol 2006;173:767-80
2005
Cheng et al. Antisense Inhibition of Human miRNAs and Indications for an Involvement of miRNA in Cell Growth and Apoptosis. Nucleic Acids Research. 2005;4:1290-1297.
Wedge et al. AZD2171: A Highly Potent, Orally Bioavailable, Vascular Endothelial Growth Factor Receptor-2 Tyrosine Kinase Inhibitor for the Treatment of Cancer. Cancer Research. 2005;65:4389-4400.
