TTPacumen

Bibliography

A selection of published posters and articles using TTP LabTech’s range of Acumen laser scanning microplate cytometers are outlined below. The PubMed links are given, where appropriate.

ARTICLES

POSTERS

2009

A high throughput screening homogenous antibody binding assay using the Acumen eX3

High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry

A novel multiplexed-compatible high throughput  cell migration screening assay using an Acumen eX3

Multiparameter high-content, high throughput analysis of the cell cycle: a comparative study using the Acumen eX3 and the ArrayScan VTI platforms

2008

HIgh content image analysis using a laser scanning  microplate cytometer

Use of far red emitting  DNA dye  DRAQ5  for cell cycle analysis with microplate cytometry

Label-free analysis of tissue sections and cells using laser scanning reflectance imaging

A high content homogneous assay for P-glycoprotein inhibition using microplate cytometry

Targeting the NFkB and M1-NFAT signling pathways using quantitiative HTS (qHTS) and cell-based ß-lactamase assays with a laser scanning microplate cytometer in 1536-well plates

 A heterogenous angiogenesis assay using primary cell co-culture in microchannels

Analysis of kinase inhibitors using a large array of signaling antibodies and high content screening

2007

Developing cell-based fluorescent assays for laser scanning microplate cytometry

Cell proliferation and cell cycole analysis using a multiple laser microplate cytometer

High content screening in oncology research  using an Acumen eX3

HIgh throughput screening using a multiple laser Acumen eX3 microplate cytometer

Cell proliferation and cell cycle analysis using a multiple laser  microplate cytometer

Cell cycle analysis using an Acumen eX3 microplate cytometer

2006

Unity of 405-nm excitable dyes in high content screening using an Acumen explorer microplate cytometer

An ultra-high throughput approach to high content screening in 1536-well format

A new high content screening paradigm: combination of image analysis software and microplate cytometry

2005

High throughput profiling of siRNA and miRNA libraries using microplate cytometry

HIgh throughout automated high content screening using a BioCube system and microplate cytometry

High content screening of red blood cell differentiation  and cytotoxicity using an Acumen Explorer

2004

Determination of protein translocation using a novel variation on the watershed algorithm

A multiplexed proliferation screening assay: differentiation and measurement of profliferation profiles in a mixed cell population using quantum dots

Multicolour detection of CD45 on human monocytes iwth quantum dots in a high-throughout format

A high content primary screening assay determining ERK adn JNK activation

 

 

ARTICLES

Karlas A et al., (2010). Genome-wide RNAi screen identifies human host factors crucial for influenza virus replcation.  Nature Jan 17 [Epub ahead of print].

Swanton C, et al., (2009). Chromosomal instability determines taxane response. Proc Natl Acad Sci U S A. 106(21):8671-6.

Brimacombe KR, et al., (2009). A dual-fluorescence high-throughput cell line system for probing multidrug resistance. Assay Drug Dev Technol. 7(3):233-49.

Auld, DS et al., (2006).  Fluorescent Protein-Based Cellular Assays Analysed by Laser Scanning Cytometry in 1536-Well Format. Methods in Enzymology (414):566-589.

Bowen, WP and Wylie, PG  (2006).  Application of laser-scanning fluorescence microplate cytometry in high content screening.  Assay and Drug Development Technologies.  4(2): 209-221.

Cheng, A.M et al., (2005)., Antisense Inhibition of Human miRNAs and Indications for an Involvement of miRNA in Cell Growth and Apoptosis. Nucleic Acids Research. (33) 4: 1290-1297.

Kittler, R et al ( 2007). Genome-scale RNAi profiling of cell division in human tissue culture cells.  Nature Cell Biology (9): 1401-1412.

Kriauciunas, A. and Roell, W., 2006.  A Perspective from Eli Lilly and Co, European Pharmaceutical Review, 11(3), p.  42-51.

Qian, Y-W., et al., 2007. Secreted PCSK9 downregulates low density lipoprotein receptor through receptor mediatiated endocytosis, Journal of Lipid Research, 48(7), p. 1488-1498.

Wedge, SR et al., (2005).  AZD2171: A Highly Potent, Orally Bioavailable, Vascular Endothelial Growth Factor Receptor-2 Tyrosine Kinase Inhibitor for the Treatment of Cancer. Cancer Research. 65(10). 4389-4400.

Wylie, P.G., and Bowen, W.P., 2007. Determination of Cell Colony Formation in a High Content Screening Assay, Clinics in Laboratory Medicine, 27(1), p. 193-9.

 

Application notes

Cell cycle analysis


 

Acumen applications

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