Bibliography
Articles | Application Notes | Posters
A selection of published posters, application notes and articles using TTP Labtech’s range of acumen microplate cytometers are outlined below. The PubMed links are given, where appropriate.
Posters
High throughput imaging of cell based assays and cellular models using microplate cytometry
Neutrophil adhesion: an HTS compatible assay using acumen eX3
High content analysis of cytotoxicity by high throughput imaging
A novel multiplexed-compatible high throughput cell migration screening assay using an acumen eX3
A heterogenous angiogenesis assay using primary cell co-culture in microchannels
Analysis of kinase inhibitors using a large array of signaling antibodies and high content screening
Application Notes
Articles
2011
Gaisford, W. Evolving methods in cytology: its the era of laser scanning, imaging-based technology. MLO, Dec 2011
Meier, T et al. Tasisulam Sodium, an Anti-tumor Agent that Inhibits Mitotic Progression and Induces Vascular Normalization. MCT, Sept 2011.
Zhao, G et al. A Novel, Selective Inhibitor of Fibroblast Growth Factor Receptors That Shows A Potent Broad-spectrum of Anti-tumor Activity in Several Tumor Xenograft Models. MCT, Sept 2011.
Kveiborg et al. 2011. PKCα and PKCδ Regulate ADAM17-Mediated Ectodomain Shedding of Heparin Binding-EGF through Separate Pathways. PloS One 6(2): e17168.
Gough et al. 2011. A quantitative, facile, and high-throughput image-based cell migration method is a robust alternative to the scratch assay. J. Biomol Screening 16(2):155-63.
Lu et al. 2011. Therapeutic targeting the loss of the birt-hogg-dube suppressor gene. Mol. Cancer. Ther. 10(1):80-9.
2010
Gupta et al. 2010. FZD4 as a Mediator of ERG Oncogene-Induced WNT Signaling and Epithelial-to-Mesenchymal Transition in Human Prostate Cancer Cells. Cancer Res. 70(17): 6735-45.
Shukla et al. 2010 . The future of toxicity testing: a focus on in vitro methods using a quantitative high-throughput screening platform. Drug Discov Today. 15(23-24):997-1007.
Chresta et al. 2010. AZD8055 is a potent, selective, and orally bioavailable ATP-competitive mammalian target of rapamycin kinase inhibitor with in vitro and in vivo antitumor activity. Cancer Res. 70(1) :288-98.
Hickinson et al. 2010. AZD8931, an Equipotent, Reversible Inhibitor of Signaling by Epidermal Growth Factor Receptor, ERBB2 (HER2), and ERBB3: A Unique Agent for Simultaneous ERBB Receptor Blockade in Cancer. Clin Cancer Res. 16:1159-69.
Fuchs et al. 2010. Clustering phenotype populations by genome-wide RNAi and multiparametric imaging. Molecular Systems Biology . 6: Article number 370
Karlas et al. 2010. Genome-wide RNAi screen identifies human host factors crucial for influenza virus replcation. Nature 463:818-822
Lees et al. 2010. P2X7 receptor-mediated killing of an intracellular parasite, toxoplasma gondii by human and murine macrophages. J Immunol. 184: 7040-6
Phong et al. 2010. The p38 MAPK promotes cell survival in response to DNA damage but is not required for G2 DNA damage checkpoint in human cancer cells. Mol Cell Biol. 30(15):3816-26.
2009
Brimacombe K.R. et al. 2009. A dual-fluorescence high-throughput cell line system for probing multidrug resistance. Assay Drug Dev. Technol. 7:233-49.
Dessole et al. 2009.Discovery of N-[(1-aryl-1H-indazol-5-yl)methyl]amides derivatives as smoothened antagonists for inhibition of the hedgehog pathway. Bioorg. Med. Chem. Lett. 19:4191-5.
Forte & Luftig 2009 MDM2-Dependent Inhibition of p53 Is Required for Epstein-Barr Virus B-Cell Growth Transformation and Infected-Cell Survival. Journal of Virology 83(6):2491-2499
Kajiyama et al. 2009. Tetracycline-inducible gene expression in conditionally immortalized mouse podocytes. Am. J. Nephrol. 29:153-163.
Klinghoffer et al. 2009. A Lentivirus-Mediated Genetic Screen Identifies Dihydrofolate Reductase (DHFR) as a Modulator of b-Catenin/GSK3 Signaling. PLoS One. 4(9). E6892.
Malony et al. 2009. Induction of thrombospondin-1 partially mediates the anti-angiogenic activity of dexrazoxane. British Journal of Cancer. 101:957–966.
Nevo et al. 2009. Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance. Clin. Cancer Res. 15:6570-81
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Salmela et al. 2009. Dietary flavonoid fisetin induces a forced exit from mitosis by targeting the mitotic spindle checkpoint. Carcinogenesis 30:1032–1040.
Swanton et al. 2009. Chromosomal instability determines taxane response. Proc Natl Acad Sci U S A. 106:8671-6.
2008
Baus et al. Identification of a novel AS160 splice variant that regulates GLUT4 translocation and glucose-uptake in rat muscle cells. Cell Signal 2008;20:2237-46
Beller et al. COPI complex is a regulator of lipid homeostasis. PloS Biology 2008;6:e292
Lehto et al. The R-Ras interaction partner ORP3 regulates cell adhesion. Journal of Cell Science 2008;121:695-705
Michael et al. A Robotic Platform for Quantitative High Throughput Screening. Assay Drug Dev Technol. 2008;6:637–657
Schmidt et al. A Novel Splicing Variant of Proprotein Convertase Subtilisin/Kexin Type 9. DNA and Cell Biology. 2008;27:183–189
Vuoriluoto et al. Syndecan-1 supports integrin α2β1-mediated adhesion to collagen. Exp Cell Res. 2008;314:3369-81
2007
Kittler et al. Genome-scale RNAi profiling of cell division in human tissue culture cells. Nature Cell Biology 2007;9:1401-12
Qian et al. Secreted PCSK9 downregulates low density lipoprotein receptor through receptor-mediated endocytosis. J Lipid Res 2007;48:1488-98
Wylie & Bowen. Determination of cell colony formation in a high-content screening assay. Clin Lab Med 2007;27:193-9
2006
Auld et al. Fluorescent Protein-Based Cellular Assays Analysed by Laser Scanning Cytometry in 1536-Well Format. Methods in Enzymology 2006;414:566-589.
Bowen & Wylie. Application of laser-scanning fluorescence microplate cytometry in high content screening. Assay and Drug Development Technologies. 2006;4: 209-221.
Kriauciunas & Roell. A Perspective from Eli Lilly and Co, European Pharmaceutical Review, 2006;11:42-51.
Pellinen et al. Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins. J Cell Biol 2006;173:767-80
