acumen hci - laser scanning imager
TTP Labtech's acumen hci is a unique laser scanning imager using up to three lasers from a range between 405 - 640 nm. Simultaneous four colour detection for each laser provides you with content-rich data. These features enable you to combine a wide range of fluorescent reagents in multicolour, multiplexed assays.
acumen hci operates by passing a laser through a widefield objective across the bottom of a microplate or slide. This allows you to rapidly analyse whole wells with resolution of 0.5 μm/pixel. The speed of data acquisition is not affected by plate density, so 24-well up to 1536-well plates (or 4 microscope slides) can be analysed at speeds of 8 minutes per plate. Uniquely, these scan times include data acquisition, data analysis and TIFF file export.
Easy entry to high-content screening
acumen’s laser scanning imaging approach makes it very easy to get into high content screening and is ideally suited to core labs. The easy-to-use software is template driven and has been designed for multiple user environments.
Because acumen's OME-compliant, whole well TIFF files are truely open source, they can be opened and further analysed in third party image analysis software. They can thus be fitted into existing workflows, complementing your existing in-house imaging expertise.
Whole well analysis
The acumen laser scanning imager is equipped with a specialised widefield objective lens which can scan entire wells and report data for all cells. This allows you to:
- overcome problems with patchy cell distribution and generates statistically robust data from heterogenous cell populations
- normalise biological responses to a total cell number
- detect rare events
- scan large objects (such as C. elegans or cell colonies) without the need for time-consuming image stitching.
Image analysis and export
acumen employs cytometric principles to rapidly obtain and analyse high quality imaging data. In addition, you can simultaneously export whole well open-source TIFF files compatible with most image analysis packages without detriment to scan times.
The acumen® hci allows you to perform a rapid primary screen of compounds or RNAis whilst exporting open-source TIFF files for subsequent secondary analysis or hit confirmation studies using existing image analysis packages; all without the need to prepare a new set of plates.
acumen® hci also lends itself to 3D culture analysis thanks to a large depth of focus and whole well imaging as standard.
This new screening paradigm represents a major breakthrough in how acumen can be applied to complex cellular models.