Laser scanning imaging with Acumen eX3
TTP LabTech's Acumen eX3 is a unique laser scanning imaging cytometer which is available in single, dual, or triple laser configurations with a choice of lasers ranging from 405nm to 633nm, ensuring compatibility with a wide variety of fluorescent probes.
This laser scanning imaging cytometer operates by passing a laser through a widefield objective across the bottom of a microplate or slide. This allows you to rapidly analyse whole wells with resolution equivalent to a 20x microscope objective. The speed of data acquisition is not affected by plate density and microplate types ranging from 24-well up to 3456-well (or 4 microscope slides) can be analysed at speeds of 6 minutes per plate. Uniquely, these scan times include data acquisition, data analysis and export.
Easy entry to high-content screening
Acumen’s laser scanning imaging approach makes it very easy to get into high content screening and is ideally suited to core labs. The easy-to-use software is template driven and has been designed for multiple user environments.
Whole well analysis
The Acumen eX3 laser scanning imaging cytometer is equipped with a specialised widefield objective lens which can scan entire wells and report data for all cells. This allows you to:
- overcome problems with patchy cell distribution and generates statistically robust data from heterogenous cell populations
- normalise biological responses to a total cell number
- detect rare events
- scan large objects (such as C. elegans or cell colonies) without the need for time-consuming image stitching
Image analysis and export
Acumen eX3 employs cytometric principles to rapidly obtain and analyse high quality imaging data. In addition, you can simultaneously export whole well open-source TIFF files compatible with most image analysis packages.
The Acumen laser scanning imaging cytometer allows you to perform a rapid primary screen of compounds or RNAis whilst exporting open-source TIFF files for subsequent secondary analysis or hit confirmation studies using existing image analysis packages; all without the need to prepare a new set of plates.
This new screening paradigm represents a major breakthrough in how Acumen can be applied to complex cellular models.
